Automatic Model Choice
Whether your model is linear or nonlinear, Multilo automatically selects the right model for you. Enter your lab readings, and it automatically selects the correct curve and fits it into your draft.
Standard Curve Generator
Change Your Assay Data into a Standard Curve for Research Paper
Make a standard curve graph in seconds with Multilo: plot your standards, fit a linear, 4PL or 5PL curve, and calculate unknown concentrations. Plus, Your curve, results, draft and lab notes stay together in one research workspace.
Watch how it works
Key takeaways
A standard curve plots the signal from samples of known concentration so you can work out the concentration of unknown samples.
The x-axis is the known concentration and the y-axis is the measured signal, such as absorbance, fluorescence or Ct value.
Use a linear fit for straight-line assays like Bradford, and a 4PL/5PL fit for S-shaped assays like ELISA.
A good curve has a high R² (close to 1) and standards that agree across replicates.
Only trust results for unknowns that fall within the range of your standards.
What sets Multilo apart
How Multilo Makes Your Standard Curve Analysis Easy
The whole process of standard curve analysis is easily done now on one platform—Multilo.
Instant solution of unknown concentrations
Enter your unknown concentrations and, within minutes, get your exact concentrations. Now there is no need to place a scale on the graph and make guesses or do calculations.
Extract Parameters without formulas
You don’t need to enter formulas manually; Mutlilo automatically gives you the intercept, R^2 value, curve equation, and slope by which your results' accuracy will be proven
Generate Graph Instantly
As you enter the data, you get the graph in seconds. This lets you analyze the axes, points, and line fit immediately.
Directly move to your document
No need to copy-paste. Your prepared graph moves directly to your .docx. File and adjusted with your writing layout
Auto writing with graph
Multilo not only makes figures but also writes descriptions so that your figures and text accurately align with each other.
Why Multilo
Graph, Write, and Cite, All in One Window
Make your standard curve, add it to your paper and cite your sources without leaving Multilo. One desktop workspace for your whole research project, and it keeps working offline
From Raw Readings to Final Graph in Four Steps
A calm, repeatable workflow for every assignment — all inside one project on your device.
Upload your data
- Attach your Excel or CSV file or select those data sets that are already present in your Multilo project.
Choose Model
- Tell in your words what kind of graph you need, or simply allow Multilo to choose a graph according to your data.
Multilo processing
- The equation, curve, slope, intercept, and R^2 value are automatically calculated from your given numbers.
Direct integration in draft
- The graph moves directly to your docx. File that is prepared for the figure legend and writing for standard curve.
What Excel and Prism Can’t Do With Your Standard Curve
Other methods also work, but they waste your time too much in formatting and increase the chance of mistakes at every next step. See how Multilo makes standard curve graphs perfectly and is the best tool for research purposes.
| Features | Multilo | Excel | GraphPad Prism | SPSS | R |
|---|---|---|---|---|---|
| Linear fit with equation, slope and R² | Automatic | Manual trendline setup | Yes | Extra steps | With code |
| 4PL / 5PL fit for ELISA | yes | Needs Solver or add-ins | Yes | Nonlinear regression setup | With packages (e.g. drc) |
| Calculates unknown concentrations | Automatic | Manual formulas | Yes | Not built in | With code |
| Graph goes straight into your document | Yes | Copy and paste | Export, then insert | Export, then insert | Export, then insert |
| Matches your draft’s formatting | Automatic | Manual | Manual | Manual | Manual |
| Coding needed | No | No | No | No | Yes |
| Cost | Free / your plan | Microsoft 365 subscription | Paid licence | Paid licence | Free |
Based on Actual Readings, Without Any Guess
A graph is as satisfactory as the accurate data behind it. Every time, Multilo passes your data through the same computational method, prepares a graph, and connects every point of your standard curve directly to your data file.
Accuracy guarantee:
All values come from your lab readings, not from any guesses, so that scientific integrity remains.
Actual and better scales:
All the small changes and differences remain in the data; it does not exaggerate your data unnecessarily or present the wrong trends.
Complete safety and privacy:
Multilo is a local desktop app, so all your data is processed only on your own device, and it is never used to train any AI model.
One-click refine
Rework any paragraph without breaking your citations
Highlight a sentence and let Multilo paraphrase, expand, or summarize it — your sources stay linked and your references stay in sync.
ParaphraseExpandSummarizeChange tone
Best for Every Type of Lab Assay and Tests
No matter the type of standard curve, Multilo works perfectly with all known standards and provides accurate results.
Protein analysis
Prepare your protein standard curve and determine unknown values from your cell lysates.
Immunoassay (ELISA or RIA)
It works best on complex standard-curve data from immunoassays such as ELISA or RIA.
Nucleic acid (DNA or RNA)
Extract correct values from the data of DNA or RNA standard curves.
Spectrophotometry and colorimetric assays
Turn your spectrophotometer’s absorbance readings from phosphate, albumin, nitrite, and other colorimetric tests into accurate concentrations instantly.
Frequently asked questions
What is a Standard Curve?
Standard curve is such a scientific graph which connects your lab readings with known concentrations. From this graph, you can find the correct and accurate concentration by seeing the reading of any unknown sample.
How Does a Standard Curve Work?
It is used in various scientific tests to measure the correct values of substances, like Bradford and BCA protein tests, qPCR, ELISA, and spectrophotometer readings.
Does Multilo make a graph from my Excel or CSV file?
Yes, you just import your file as a CSV or EXCEL and explain in simple words what you want, and it generates a graph automatically from your actual standards.
Is my assay data safe and private?
Yes, your data runs locally on your desktop, and it remains on your device. It is never used to train any AI model.
Is there any limit to processing data?
No, there is no limit. You can process as much data as you want, but if the dataset is too large, then it may take some more time.
Does Multilo give me the curve equation and R^2 value?
Yes, Multilo automatically extracts the R^2 value, slope, and equation so that you can report it directly in your research draft.
Can I use it for Bradford, BCA, or BSA assay?
Yes, enter your known standards and readings; Multilo calculates your unknown sample concentration within seconds by making a curve.
Plot Graph and Start Writing Your Draft
No need for making templates repeatedly and copy-pasting screenshots. Download Multilo, import your standards, get a complete description, and prepare a standard curve within your research paper or document in seconds.